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Glutathione Articles - COPD (Chronic Obstructive Pulmonary Disease) & Lung Disorders
Depletion of Glutathione S-transferase P1 Induces Apoptosis in Human Lung
Fibroblasts
Ishii T, Fujishiro M, Masuda M, Nakajima J,
Teramoto S, Ouchi Y, Matsuse T
Exp Lung Res. 2003 Oct-Nov;29(7):523-36
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Glutathione S-transferase P1
(GSTP1) is one of the xenobiotic-metabolizing and antioxidant enzymes,
identified in the peripheral lungs. Recently, the authors reported the
association between GSTP1 gene polymorphism and susceptibility to chronic
obstructive pulmonary disease (COPD), and protective effect of GSTP1
against cigarette smoke in human lung fibroblasts in vitro. In this study,
the authors investigated that depletion of GSTP1 by itself could induce
cell death,
including apoptosis, in human lung fibroblast-derived HFL-1 cells. The
level of apoptosis and necrosis was increased significantly with GSTP1
antisense vector transfection. It was also observed that the transfection
efficiency and the expression level of the vector were weaker in the
transfectant of the antisense vector than in those of the sense and
control vectors, which is also thought to indicate that inhibition of
GSTP1 expression by the antisense vector alone affects cellular viability.
However, there was no difference among these transfectants neither on
glutathione (GSH) level nor on c-Jun NH2-terminal kinase (JNK) activation.
Therefore, the authors report here that underexpression of GSTP1 appeared
to induce apoptosis on lung fibroblasts, which suggests that GSTP1 may
have protective effects against apoptosis in the airway cells, though the
mechanism of this apoptotic pathway is still to be elucidated.
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